BT6212 MICROBIOLOGY LAB Syllabus | Anna University Regulation 2013 BE/B Tech First Year Second Semester Syllabus. Below is the Anna University 2013 Regulation Syllabus for 2nd Semester for Biotechnology, Textbooks, Reference books, Exam portions, Question Bank, Previous year question papers, Model question papers, Class notes, Important 2 marks, 8 marks, 16 marks topics. It is applicable for all students admitted in the Academic year 2013-2014 onwards for all its Affiliated institutions in Tamil nadu.
1. Introduction, Laboratory Safety, Use of Equipment; Sterilization Techniques;
2. Culture Media-Types and Use; Preparation of Nutrient broth and agar
3. Culture Techniques, Isolation and Preservation of Cultures- Broth: flask, test
tubes; Solid: Pour plates, streak plates, slants, stabs
4. Microscopy – Working and care of Microscope
5. Microscopic Methods in the Study of Microorganisms; Staining Techniques-
Simple, Differential- Gram’s Staining
6. Quantification of Microbes: Sampling and Serial Dilution; Bacterial count in Soil – TVC
7. Effect of Disinfectants- Phenol Coefficient
8. Antibiotic Sensitivity Assay
9. Growth Curve in Bacteria and Yeast
10. Effect of pH, Temperature, UV radiation on Growth Bacteria
TOTAL: 60 PERIODS
Equipment Needed for 20 Students
Autocalve 1
Hot Air Oven 1
Incubators 2
Light Microscopes 4
Incubator Shaker 1
Colorimeter 2
Lamina Flow Chamber 2
Glassware, Chemicals, Media as required
10
REFERENCE BOOKS
1. Cappuccino, J.G. and N. Sherman “Microbiology : A Laboratory Manual”, 4th Edition, Addison-
Wesley, 1999.
2. Collee, J.G. etal., “Mackie & McCartney Practical Medical Microbiology” 4th Edition, Churchill
Livingstone, 1996.
BT6212 MICROBIOLOGY LABORATORY L T P C 0 0 4 2
Experiments1. Introduction, Laboratory Safety, Use of Equipment; Sterilization Techniques;
2. Culture Media-Types and Use; Preparation of Nutrient broth and agar
3. Culture Techniques, Isolation and Preservation of Cultures- Broth: flask, test
tubes; Solid: Pour plates, streak plates, slants, stabs
4. Microscopy – Working and care of Microscope
5. Microscopic Methods in the Study of Microorganisms; Staining Techniques-
Simple, Differential- Gram’s Staining
6. Quantification of Microbes: Sampling and Serial Dilution; Bacterial count in Soil – TVC
7. Effect of Disinfectants- Phenol Coefficient
8. Antibiotic Sensitivity Assay
9. Growth Curve in Bacteria and Yeast
10. Effect of pH, Temperature, UV radiation on Growth Bacteria
TOTAL: 60 PERIODS
Equipment Needed for 20 Students
Autocalve 1
Hot Air Oven 1
Incubators 2
Light Microscopes 4
Incubator Shaker 1
Colorimeter 2
Lamina Flow Chamber 2
Glassware, Chemicals, Media as required
10
REFERENCE BOOKS
1. Cappuccino, J.G. and N. Sherman “Microbiology : A Laboratory Manual”, 4th Edition, Addison-
Wesley, 1999.
2. Collee, J.G. etal., “Mackie & McCartney Practical Medical Microbiology” 4th Edition, Churchill
Livingstone, 1996.
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