BT6211 BIOCHEMISTRY LAB Syllabus | Anna University Regulation 2013 BE/B Tech First Year Second Semester Syllabus. Below is the Anna University 2013 Regulation Syllabus for 2nd Semester for Biotechnology, Textbooks, Reference books, Exam portions, Question Bank, Previous year question papers, Model question papers, Class notes, Important 2 marks, 8 marks, 16 marks topics. It is applicable for all students admitted in the Academic year 2013-2014 onwards for all its Affiliated institutions in Tamil nadu.
To learn and understand the principles behind the qualitative and quantitative estimation of
biomolecules (proteins, carbohydrates, lipids, metabolites etc.,) and laboratory analysis of
the same in the body fluids.
EXPERIMENTS
1. Preparation and measurement of pH of standard buffers (phosphate, carbonate, borate, TRIS
etc.,).
2. Qualitative analysis of carbohydrates (monosaccharides, disaccharides, polysaccharides etc.,)
3. Enzymatic hydrolysis of glycogen by α and β amylase
4. Qualitative analysis of proteins
9
5. Qualitative analysis of lipids (triglycerides, cholesterol, phospholipids etc.,)
6. Quantitative analysis of proteins (Lowrys method, Bradford, UV)
7. Quantitative analysis of carbohydrates (Benedict’s method etc.,) lipids
8. Quantitative analysis of lipids (Benedict’s method etc.,)
9. Quantitative estimation of blood glucose
10. Acid hydrolysis and action of salivary amylase on starch
11. Estimation of chloride, glucose, ammonia and creatinine in urine.
12. Quantitative analysis of urea in serum
13. Quantitative analysis of serum bilirubin
14. Quantitative estimation of serum cholesterol by Libermann Burchard’s method
15. Isolation and assay of glycogen from the liver and skeletal muscle of mice
TOTAL: 60 PERIODS
Equipment Required
1. UV-Visible Spectrophotometers
2. pH meter
3. Centrifuge
TEXT BOOKS
1. Gupta R.C. and Bhargavan S. Practical Biochemistry.
2. David T. Phummer. Introduction of Practical Biochemistry (II Edition).
REFERENCES
1. Murray R.K., Granner D.K., Mayes P.A. and Rodwell V.W. Harpers Biochemistry, Appleton
and Lange ,Stanford ,Conneticut.
2. Thomas M. Devlin. Textbook of Biochemistry with clinical correlations. Wiley Liss Publishers
BT6211 BIOCHEMISTRY LABORATORY L T P C 0 0 4 2
OBJECTIVES To learn and understand the principles behind the qualitative and quantitative estimation of
biomolecules (proteins, carbohydrates, lipids, metabolites etc.,) and laboratory analysis of
the same in the body fluids.
EXPERIMENTS
1. Preparation and measurement of pH of standard buffers (phosphate, carbonate, borate, TRIS
etc.,).
2. Qualitative analysis of carbohydrates (monosaccharides, disaccharides, polysaccharides etc.,)
3. Enzymatic hydrolysis of glycogen by α and β amylase
4. Qualitative analysis of proteins
9
5. Qualitative analysis of lipids (triglycerides, cholesterol, phospholipids etc.,)
6. Quantitative analysis of proteins (Lowrys method, Bradford, UV)
7. Quantitative analysis of carbohydrates (Benedict’s method etc.,) lipids
8. Quantitative analysis of lipids (Benedict’s method etc.,)
9. Quantitative estimation of blood glucose
10. Acid hydrolysis and action of salivary amylase on starch
11. Estimation of chloride, glucose, ammonia and creatinine in urine.
12. Quantitative analysis of urea in serum
13. Quantitative analysis of serum bilirubin
14. Quantitative estimation of serum cholesterol by Libermann Burchard’s method
15. Isolation and assay of glycogen from the liver and skeletal muscle of mice
TOTAL: 60 PERIODS
Equipment Required
1. UV-Visible Spectrophotometers
2. pH meter
3. Centrifuge
TEXT BOOKS
1. Gupta R.C. and Bhargavan S. Practical Biochemistry.
2. David T. Phummer. Introduction of Practical Biochemistry (II Edition).
REFERENCES
1. Murray R.K., Granner D.K., Mayes P.A. and Rodwell V.W. Harpers Biochemistry, Appleton
and Lange ,Stanford ,Conneticut.
2. Thomas M. Devlin. Textbook of Biochemistry with clinical correlations. Wiley Liss Publishers
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