BT2026 BIOCONJUGATE TECHNOLOGY SYLLABUS | ANNA UNIVERSITY BTECH BIOTECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SIXTH SEMESTER B.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2026 BIOCONJUGATE TECHNOLOGY L T P C
3 0 0 3
AIM
To develop the skills of Student in the area of Bio conjugate technology and its industrial
applications.
OBJECTIVES
At the end of the course, the student would have learnt about enzymes, nucleic acids
and how to modify them for target specificity. Student also gets familiarized with the
industrial applications of this technology.
UNIT I FUNCTIONAL TARGETS 9
Modification of Amino Acids, Peptides and Proteins – Modification of sugars,
polysaccharides and glycoconjugates – modification of nucleic acids and
oligonucleotides.
UNIT II CHEMISTRY OF ACIVE GROUPS 9
Amine reactive chemical reactions – Thiol reactive chemical reactions – carboxylate
reactive chemical reactions – hydroxyl reactive chemical reactions – aldehyde and
ketone reactive chemical reactions – Photoreactive chemical reactions.
UNIT III BIOCONJUGATE REAGENTS 9
Zero length cross linkers – Homobifunctional cross linkers – Heterobifunctional cross
linkers – Trifunctional cross linkers – Cleavable reagent systems – tags and probes.
UNIT IV ENZYME AND NUCLEIC ACID MODIFICATION AND CONJUGATION
9
Properties of common enzymes – Activated enzymes for conjugation – biotinylated
enzymes – chemical modification of nucleic acids – biotin labeling of DNA- enzyme
conjugation to DNA – Fluorescent of DNA.
UNIT V BIOCONJUGATE APLICATIONS 9
Preparation of Hapten-carrier Immunogen conjugates - antibody modification and
conjugation – immunotoxin conjugation techniques – liposome conjugated and
derivatives- Colloidal – gold-labeled proteins – modification with synthetic polymers.
TOTAL : 45 PERIODS
TEXT BOOK
1. Bioconjugate Techniques, G.T. Hermanson, Academic Press, 1999.
BT2026 BIOCONJUGATE TECHNOLOGY L T P C
3 0 0 3
AIM
To develop the skills of Student in the area of Bio conjugate technology and its industrial
applications.
OBJECTIVES
At the end of the course, the student would have learnt about enzymes, nucleic acids
and how to modify them for target specificity. Student also gets familiarized with the
industrial applications of this technology.
UNIT I FUNCTIONAL TARGETS 9
Modification of Amino Acids, Peptides and Proteins – Modification of sugars,
polysaccharides and glycoconjugates – modification of nucleic acids and
oligonucleotides.
UNIT II CHEMISTRY OF ACIVE GROUPS 9
Amine reactive chemical reactions – Thiol reactive chemical reactions – carboxylate
reactive chemical reactions – hydroxyl reactive chemical reactions – aldehyde and
ketone reactive chemical reactions – Photoreactive chemical reactions.
UNIT III BIOCONJUGATE REAGENTS 9
Zero length cross linkers – Homobifunctional cross linkers – Heterobifunctional cross
linkers – Trifunctional cross linkers – Cleavable reagent systems – tags and probes.
UNIT IV ENZYME AND NUCLEIC ACID MODIFICATION AND CONJUGATION
9
Properties of common enzymes – Activated enzymes for conjugation – biotinylated
enzymes – chemical modification of nucleic acids – biotin labeling of DNA- enzyme
conjugation to DNA – Fluorescent of DNA.
UNIT V BIOCONJUGATE APLICATIONS 9
Preparation of Hapten-carrier Immunogen conjugates - antibody modification and
conjugation – immunotoxin conjugation techniques – liposome conjugated and
derivatives- Colloidal – gold-labeled proteins – modification with synthetic polymers.
TOTAL : 45 PERIODS
TEXT BOOK
1. Bioconjugate Techniques, G.T. Hermanson, Academic Press, 1999.
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