PY3312 SEPARATION TECHNOLOGY SYLLABUS | ANNA UNIVERSITY BTECH PHARMACEUTICAL TECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SIXTH SEMESTER B.TECH PHARMACEUTICAL TECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
PY3312 SEPARATION TECHNOLOGY
UNIT I BIO-SEPARATION 9
Characterization of biomolecules – Fermentation broths – Role of downstream
processing in biotechnology – Broad strategies for design of bio-separation processes.
UNIT II DOWNSTREAM PROCESSING 9
Cell disruption – Mechanical and chemical methods – Cake filtration and microfiltration –
Centrifugation and sedimentation – Membrane processes – Dialysis – Ultra filtration –
Reverse osmosis and electro-dialysis.
UNIT III LIQUID-LIQUID SEPARATIONS AND PROTEIN SEPARATIONS 9
Solvent extraction of small molecules – Aqueous two-phase extraction of proteins –
PEG/Dextran separation – Precipitation of proteins with salts and organic solvents –
Salting out method – Electrostatic interaction – Protein solvation with polyelectrolytes –
Polyvalent metal ions – Sorption.
UNIT IV CHROMATOGRAPHIC SEPARATIONS 9
Adsorption processes – Principles of chromatographic separation – Gel filtration –
Reversed phase – Hydrophobic interaction – Ion-exchange – Immuno-bio-affinity
chromatography – Design and selection of chromatographic matrices – Modes of
operations – Design of large scale chromatographic separation processes –
Electrophoresis.
UNIT V PRODUCT PURIFICATION 9
Crystallization – Crystal geometry – Equilibrium and yields – Nucleation – Crystal growth
– Equipments for crystallization – Continuous crystallizer – Draft tube baffle crystallizer –
Drying and lyophillization – Principles – Temperature patterns – Heat transfer in dryers –
Bound –Unbound water – Freeze drying or lyophillization – Equipments for drying – Tray
dryer – Fluidised bed dryer – Spray dryer.
TOTAL: 45 PERIODS
TEXTBOOKS
1. Geankoplis, C.J., “Transport Processes and Separation Process Principles”, 4th
Edition, Prentice-Hall, Inc, 2003.
2. Mukhopadhyay, S.N., “Process Biotechnology Fundamentals”, 2nd Edition, Viva
Books (P) Ltd., 2004.
L T P C
3 0 0 3
50
REFERENCES
1. Adamovies, J.A., “Chromatographic Analysis of Pharmaceuticals”, 2nd Edition,
Marcel Dekker, 1997.
2. McCabe, W.L., Smith, J.C and Harriot, P., “Unit Operations of Chemical
Engineering”, 6th Edition, McGraw Hill Publications, 2001.
3. Cruger, W. and Cruger,V., “A Text Book of Industrial Microbiology”, 2nd Edition,
Prentice-Hall, Inc, 1997.
PY3312 SEPARATION TECHNOLOGY
UNIT I BIO-SEPARATION 9
Characterization of biomolecules – Fermentation broths – Role of downstream
processing in biotechnology – Broad strategies for design of bio-separation processes.
UNIT II DOWNSTREAM PROCESSING 9
Cell disruption – Mechanical and chemical methods – Cake filtration and microfiltration –
Centrifugation and sedimentation – Membrane processes – Dialysis – Ultra filtration –
Reverse osmosis and electro-dialysis.
UNIT III LIQUID-LIQUID SEPARATIONS AND PROTEIN SEPARATIONS 9
Solvent extraction of small molecules – Aqueous two-phase extraction of proteins –
PEG/Dextran separation – Precipitation of proteins with salts and organic solvents –
Salting out method – Electrostatic interaction – Protein solvation with polyelectrolytes –
Polyvalent metal ions – Sorption.
UNIT IV CHROMATOGRAPHIC SEPARATIONS 9
Adsorption processes – Principles of chromatographic separation – Gel filtration –
Reversed phase – Hydrophobic interaction – Ion-exchange – Immuno-bio-affinity
chromatography – Design and selection of chromatographic matrices – Modes of
operations – Design of large scale chromatographic separation processes –
Electrophoresis.
UNIT V PRODUCT PURIFICATION 9
Crystallization – Crystal geometry – Equilibrium and yields – Nucleation – Crystal growth
– Equipments for crystallization – Continuous crystallizer – Draft tube baffle crystallizer –
Drying and lyophillization – Principles – Temperature patterns – Heat transfer in dryers –
Bound –Unbound water – Freeze drying or lyophillization – Equipments for drying – Tray
dryer – Fluidised bed dryer – Spray dryer.
TOTAL: 45 PERIODS
TEXTBOOKS
1. Geankoplis, C.J., “Transport Processes and Separation Process Principles”, 4th
Edition, Prentice-Hall, Inc, 2003.
2. Mukhopadhyay, S.N., “Process Biotechnology Fundamentals”, 2nd Edition, Viva
Books (P) Ltd., 2004.
L T P C
3 0 0 3
50
REFERENCES
1. Adamovies, J.A., “Chromatographic Analysis of Pharmaceuticals”, 2nd Edition,
Marcel Dekker, 1997.
2. McCabe, W.L., Smith, J.C and Harriot, P., “Unit Operations of Chemical
Engineering”, 6th Edition, McGraw Hill Publications, 2001.
3. Cruger, W. and Cruger,V., “A Text Book of Industrial Microbiology”, 2nd Edition,
Prentice-Hall, Inc, 1997.
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