IB3312 GENETIC ENGINEERING SYLLABUS | ANNA UNIVERSITY BTECH FOOD TECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SIXTH SEMESTER B.TECH FOOD TECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
IB3312 GENETIC ENGINEERING L T P C
3 0 0 3
UNIT I BASICS OF RECOMBINANT DNA TECHNOLOGY 4
Role of genes within cells, genetic elements that control gene expression, restriction and
modifying enzymes, safety guidelines of recombinant DNA research.
UNIT II CREATION OF RECOMBINANT MOLECULES 10
Restriction mapping, design of linkers and adaptors. Characteristics of plasmid and phage
vectors, prokaryotic and eukaryotic expression vectors. Insect, Yeast and Mammalian
vectors.
UNIT III CONSTRUCTION OF LIBRARIES 15
Construction of cDNA and genomic libraries. Screening of libraries with DNA probes and
with antisera.
45
UNIT IV POLYMERASE CHAIN REACTION 10
Inverse PCR, Nested PCR, Taqman assay, Molecular beacons, RACE PCR, RAPD, site
directed mutagenesis, methods of nucleic acid sequencing- Sangers method, (Kunkel’s
Method).
UNIT V APPLICATIONS OF RECOMBINANT DNA TECHNOLOGY 6
Cloning in plants, Ti plasmid, and transgenic and knockout animals.
TOTAL : 45 PERIODS
TEXT BOOKS
1. Primrose, S.Twyman, R. “Principles of Gene Manipulation and Genomics” 7th Edition,
Blackwell Publishing, 2006.
2. Brown, T.A. “Gene Cloning & DNA Analysis : An Introduction”, 5th Edition, Blackwell
Publishing, 2006.
3. Watson, James., Molecular Biology of the Gene” 5ht Edition, Pearson Education,2004.
4. Glick, B.R. and J.J. Pasternak “Molecular Biotechnology : Principles and Applications of
Recombinant DNA”, 3rd Edition, ASM, 2003.
REFERENCES
1. Winnacker, Ernst – L. “From Genes to Clones : Introduction to Gene Technology”,
Panima, 2003.
2. Karp, Gerald. “Cell and Molecular Biology : Concepts and Experiments”, 4th Edition, John
– Wiley & Sons, 2005.
3. Mc Pherson, M.J. and S.G. Moller “PCR” Bios Scientific Publication, 2000.
4. Hughes, S. and Moody, “PCR” Scion Publishing Ltd., 2007.
IB3312 GENETIC ENGINEERING L T P C
3 0 0 3
UNIT I BASICS OF RECOMBINANT DNA TECHNOLOGY 4
Role of genes within cells, genetic elements that control gene expression, restriction and
modifying enzymes, safety guidelines of recombinant DNA research.
UNIT II CREATION OF RECOMBINANT MOLECULES 10
Restriction mapping, design of linkers and adaptors. Characteristics of plasmid and phage
vectors, prokaryotic and eukaryotic expression vectors. Insect, Yeast and Mammalian
vectors.
UNIT III CONSTRUCTION OF LIBRARIES 15
Construction of cDNA and genomic libraries. Screening of libraries with DNA probes and
with antisera.
45
UNIT IV POLYMERASE CHAIN REACTION 10
Inverse PCR, Nested PCR, Taqman assay, Molecular beacons, RACE PCR, RAPD, site
directed mutagenesis, methods of nucleic acid sequencing- Sangers method, (Kunkel’s
Method).
UNIT V APPLICATIONS OF RECOMBINANT DNA TECHNOLOGY 6
Cloning in plants, Ti plasmid, and transgenic and knockout animals.
TOTAL : 45 PERIODS
TEXT BOOKS
1. Primrose, S.Twyman, R. “Principles of Gene Manipulation and Genomics” 7th Edition,
Blackwell Publishing, 2006.
2. Brown, T.A. “Gene Cloning & DNA Analysis : An Introduction”, 5th Edition, Blackwell
Publishing, 2006.
3. Watson, James., Molecular Biology of the Gene” 5ht Edition, Pearson Education,2004.
4. Glick, B.R. and J.J. Pasternak “Molecular Biotechnology : Principles and Applications of
Recombinant DNA”, 3rd Edition, ASM, 2003.
REFERENCES
1. Winnacker, Ernst – L. “From Genes to Clones : Introduction to Gene Technology”,
Panima, 2003.
2. Karp, Gerald. “Cell and Molecular Biology : Concepts and Experiments”, 4th Edition, John
– Wiley & Sons, 2005.
3. Mc Pherson, M.J. and S.G. Moller “PCR” Bios Scientific Publication, 2000.
4. Hughes, S. and Moody, “PCR” Scion Publishing Ltd., 2007.
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