FT3319 GENETIC ENGINEERING LAB SYLLABUS | ANNA UNIVERSITY BTECH FOOD TECHNOLOGY 6TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SIXTH SEMESTER B.TECH FOOD TECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
FT3319 GENETIC ENGINEERING LAB L T P C
0 0 4 2
1. Preparation of plasmid DNA
2. Elution of DNA from agarose gels
3. Ligation of DNA into expression vectors
4. Transformation
5. Optimisation of inducer concentration for recombinant protein expression
6. Optimisation of time of inducer for recombinant protein expression
7. SDS-PAGE
8. Western blotting
9. Hybridisation with anti-sera
10. PCR.
TOTAL : 60 PERIODS
REFERENCE
1. Sambrook, Joseph and David W. Russell “The Condensed Protocols : From Molecular
Cloning ; A Laboratory Manual” Cold Spring Harbor Laboratory Press, 2006.
FT3319 GENETIC ENGINEERING LAB L T P C
0 0 4 2
1. Preparation of plasmid DNA
2. Elution of DNA from agarose gels
3. Ligation of DNA into expression vectors
4. Transformation
5. Optimisation of inducer concentration for recombinant protein expression
6. Optimisation of time of inducer for recombinant protein expression
7. SDS-PAGE
8. Western blotting
9. Hybridisation with anti-sera
10. PCR.
TOTAL : 60 PERIODS
REFERENCE
1. Sambrook, Joseph and David W. Russell “The Condensed Protocols : From Molecular
Cloning ; A Laboratory Manual” Cold Spring Harbor Laboratory Press, 2006.
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