BT9231 ADVANCED MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER M.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2009 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT9231 ADVANCED MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB L T P C
0 0 6 3
1. Preparation of Genomic DNA
2. PCR amplification of gene from the genomic DNA
3. Preparation of plasmid DNA
4. Restriction Digestion of the vector and Insert
5. Ligation and Transformation to E.coli
6. Lysate PCR confirmation.
7. Restriction & gel elution of DNA fragments
8. Electroporation to Yeast
9. Induction experiments in E.coli using IPTG, salt etc
10. SDS-PAGE analysis of expression
11. Western blot confirmation of expressed protein (anti his)
12. ELISA (anti his) – Quantification of expressed protein.
13. RNA Isolation
14. cDNA preparation from RNA
15. Site directed mutagenesis
16. Southern hybridization experiment
TOTAL : 90 PERIODS
BT9231 ADVANCED MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB L T P C
0 0 6 3
1. Preparation of Genomic DNA
2. PCR amplification of gene from the genomic DNA
3. Preparation of plasmid DNA
4. Restriction Digestion of the vector and Insert
5. Ligation and Transformation to E.coli
6. Lysate PCR confirmation.
7. Restriction & gel elution of DNA fragments
8. Electroporation to Yeast
9. Induction experiments in E.coli using IPTG, salt etc
10. SDS-PAGE analysis of expression
11. Western blot confirmation of expressed protein (anti his)
12. ELISA (anti his) – Quantification of expressed protein.
13. RNA Isolation
14. cDNA preparation from RNA
15. Site directed mutagenesis
16. Southern hybridization experiment
TOTAL : 90 PERIODS
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