BT 9232 ADVANCED BIOPROCESS AND DOWNSTREAM PROCESSING LAB SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER M.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2009 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT 9232 ADVANCED BIOPROCESS AND DOWNSTREAM PROCESSING LAB L T P C
0 0 6 3
1. Enzyme kinetics, inhibition, factors affecting reaction ph, temp.
2. Enzyme immobilization studies – Gel entrapment, adsorption and ion exchange
immobilisation.
3. Optimization techniques – Plackett burman, Response surface methodology.
4. Batch cultivation – recombinant E.coli – growth rate, substrate utilization kinetics,
plasmid stability, product analysis after induction, Metabolite analysis by HPLC
5. Fed batch cultivation E.coli, Pichia pastoris
6. Continuous cultivation – x - d construction, kinetic parameter evaluation, gas
analysis, carbon balancing, Pulse and shift techniques.
7. Bioreactor studies : Sterlisation kinetics, kLa determination, residence time
distribution
8. Animal cell culture production: T-flask, spinner flask, bioreactor
9. Cell separation methods; Centrifugation and microfiltration
10. Cell disruption methos: Chemical lysis and Physical methods
11. Product concentration: Precipitation, ATPS, Ultrafiltration
12. High resolution purification; Ion exchange, affinity and Gel filtration
13. Freeze drying
TOTAL : 90 PERIODS
BT 9232 ADVANCED BIOPROCESS AND DOWNSTREAM PROCESSING LAB L T P C
0 0 6 3
1. Enzyme kinetics, inhibition, factors affecting reaction ph, temp.
2. Enzyme immobilization studies – Gel entrapment, adsorption and ion exchange
immobilisation.
3. Optimization techniques – Plackett burman, Response surface methodology.
4. Batch cultivation – recombinant E.coli – growth rate, substrate utilization kinetics,
plasmid stability, product analysis after induction, Metabolite analysis by HPLC
5. Fed batch cultivation E.coli, Pichia pastoris
6. Continuous cultivation – x - d construction, kinetic parameter evaluation, gas
analysis, carbon balancing, Pulse and shift techniques.
7. Bioreactor studies : Sterlisation kinetics, kLa determination, residence time
distribution
8. Animal cell culture production: T-flask, spinner flask, bioreactor
9. Cell separation methods; Centrifugation and microfiltration
10. Cell disruption methos: Chemical lysis and Physical methods
11. Product concentration: Precipitation, ATPS, Ultrafiltration
12. High resolution purification; Ion exchange, affinity and Gel filtration
13. Freeze drying
TOTAL : 90 PERIODS
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