BT 9222 ADVANCED GENETIC ENGINEERING SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER M.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2009 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT 9222 ADVANCED GENETIC ENGINEERING L T P C
3 0 0 3
UNIT I CLONING AND EXPRESSION OF GENES 10
Cloning vehicles, restriction enzymes, restriction modification, linkers, adaptors,
homopolymeric trailing, restriction mapping
Expression and purification of recombinant proteins, prokaryotic and eukaryotic
expression vectors, in vivo homologous recombination, large scale expression and
purification of proteins.
UNIT II LIBRARY CONSTRUCTION 8
cDNA & genomic DNA library construction and screening, preparation of DNA, RNA
probes immunoscreening and blotting techniques, etc
10
UNIT III SEQUENCING 10
Methodology – Chemical & enzymatic, Automated sequence, Genome sequencing
methods – top down approach, bottom up approach.
UNIT IV PCR AND MUTAGENESIS 7
PCR principle, applications, different types of PCR, mutagenesis and chmeric protein
engineering by PCR, RACE, Kuntels’ method of mutagenesis.
UNIT V GENE TRANSFER & GENE THERAPY 10
Introduction of foreign genes into plant and animal cells, creation of transgenic plants
and animal knockouts, gene therapy, types and vectors.
TOTAL : 45 PERIODS
REFERENCES
1. Primrose S.B.., Twyman R.H. and Old R.W. Principles of Gene Manipulation, 6th ed.,
Blackwell Science, 2001
2. Winnacker E.L. Frome Genes to clones : Introduction to Gene Technology, Panima,
2003
3. Glick B.R. and Pasternak J.J. Molecular Biotechnology: Principles and applications
of recombinant DNA, 3rd ed., ASM Press, 2003
4. Lemonie, N.R. and Cooper, D.N. Gene therapy, BIOS Scientific, 1996
BT 9222 ADVANCED GENETIC ENGINEERING L T P C
3 0 0 3
UNIT I CLONING AND EXPRESSION OF GENES 10
Cloning vehicles, restriction enzymes, restriction modification, linkers, adaptors,
homopolymeric trailing, restriction mapping
Expression and purification of recombinant proteins, prokaryotic and eukaryotic
expression vectors, in vivo homologous recombination, large scale expression and
purification of proteins.
UNIT II LIBRARY CONSTRUCTION 8
cDNA & genomic DNA library construction and screening, preparation of DNA, RNA
probes immunoscreening and blotting techniques, etc
10
UNIT III SEQUENCING 10
Methodology – Chemical & enzymatic, Automated sequence, Genome sequencing
methods – top down approach, bottom up approach.
UNIT IV PCR AND MUTAGENESIS 7
PCR principle, applications, different types of PCR, mutagenesis and chmeric protein
engineering by PCR, RACE, Kuntels’ method of mutagenesis.
UNIT V GENE TRANSFER & GENE THERAPY 10
Introduction of foreign genes into plant and animal cells, creation of transgenic plants
and animal knockouts, gene therapy, types and vectors.
TOTAL : 45 PERIODS
REFERENCES
1. Primrose S.B.., Twyman R.H. and Old R.W. Principles of Gene Manipulation, 6th ed.,
Blackwell Science, 2001
2. Winnacker E.L. Frome Genes to clones : Introduction to Gene Technology, Panima,
2003
3. Glick B.R. and Pasternak J.J. Molecular Biotechnology: Principles and applications
of recombinant DNA, 3rd ed., ASM Press, 2003
4. Lemonie, N.R. and Cooper, D.N. Gene therapy, BIOS Scientific, 1996
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