PY3217 BIOCHEMISTRY LAB SYLLABUS | ANNA UNIVERSITY BTECH PHARMACEUTICAL TECHNOLOGY 4TH SEM SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FOURTH SEMESTER B TECH PHARMACEUTICAL TECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
PY3217 BIOCHEMISTRY LABORATORY
1. Study of units for volume, weight, measurements and concentration. sensitivity,
specificity, precision and accuracy.
2. Preparation of buffers and pH measurements.
3. Qualitative tests for carbohydrates.
4. Qualitative tests for amino acids.
5. Estimation of Reducing sugars by the Benedict’s method.
6. Estimation of amino acids by ninhydrin method.
7. Protein estimation by various methods.
8. Acid hydrolysis of proteins
9. Enzyme assays: Phosphatase from potato, Amylase from sweet potato, Trypsin
digestion of proteins, Assay of proteases, NADH Dehydrogenase, Catalase and
peroxidase.
10. Extraction of lipids.
11. Phospholipids: Ashing and estimation of phosphate.
12. Estimation of cholesterol.
13. Estimation of nucleic acids.
TOTAL: 45 PERIODS
PY3217 BIOCHEMISTRY LABORATORY
1. Study of units for volume, weight, measurements and concentration. sensitivity,
specificity, precision and accuracy.
2. Preparation of buffers and pH measurements.
3. Qualitative tests for carbohydrates.
4. Qualitative tests for amino acids.
5. Estimation of Reducing sugars by the Benedict’s method.
6. Estimation of amino acids by ninhydrin method.
7. Protein estimation by various methods.
8. Acid hydrolysis of proteins
9. Enzyme assays: Phosphatase from potato, Amylase from sweet potato, Trypsin
digestion of proteins, Assay of proteases, NADH Dehydrogenase, Catalase and
peroxidase.
10. Extraction of lipids.
11. Phospholipids: Ashing and estimation of phosphate.
12. Estimation of cholesterol.
13. Estimation of nucleic acids.
TOTAL: 45 PERIODS
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