PY3216 MICROBIOLOGY LAB SYLLABUS | ANNA UNIVERSITY BTECH PHARMACEUTICAL TECHNOLOGY 4TH SEM SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FOURTH SEMESTER B TECH PHARMACEUTICAL TECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
PY3216 MICROBIOLOGY LABORATORY
1. Sterilization techniques (lecture / demonstration)
2. Preparation of various culture media.
3. Culturing of micro-organisms
(a) Isolation of specific group of microbes symbiotic – asymbiotic,
chemolithotrophs, organotrophs
(b) Pure culture techniques – Streak plate, pour plate, isolation and preservation
of bacterial culture, single spore isolation.
4. Identification of microorganisms by staining techniques
5. Identification of microorganisms by biochemical testing.
6. Quantification of microorganisms by turbidimetry
7. Quantification of microorganisms by serial dilution, MPM.
8. Preservation of cells, slants stabs, use of mineral oil, liquid paraffin, whey,
glycerol, sterile water, lyophilization.
9. Environmental sample analysis – Polluted soil and effluent.
10. Food microbiology – Milk – Fermented food – Yogurt, meat, ice cream
11. Clinical microbiology – Normal mouth flora, pus, urine.
12. Determination of MIC of antimicrobial agents
TOTAL: 45 PERIODS
PY3216 MICROBIOLOGY LABORATORY
1. Sterilization techniques (lecture / demonstration)
2. Preparation of various culture media.
3. Culturing of micro-organisms
(a) Isolation of specific group of microbes symbiotic – asymbiotic,
chemolithotrophs, organotrophs
(b) Pure culture techniques – Streak plate, pour plate, isolation and preservation
of bacterial culture, single spore isolation.
4. Identification of microorganisms by staining techniques
5. Identification of microorganisms by biochemical testing.
6. Quantification of microorganisms by turbidimetry
7. Quantification of microorganisms by serial dilution, MPM.
8. Preservation of cells, slants stabs, use of mineral oil, liquid paraffin, whey,
glycerol, sterile water, lyophilization.
9. Environmental sample analysis – Polluted soil and effluent.
10. Food microbiology – Milk – Fermented food – Yogurt, meat, ice cream
11. Clinical microbiology – Normal mouth flora, pus, urine.
12. Determination of MIC of antimicrobial agents
TOTAL: 45 PERIODS
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