BT2258 INSTRUMENTAL METHODS OF ANALYSIS LAB SYLLABUS | ANNA UNIVERSITY BTECH BIOTECHNOLOGY 4TH SEM SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FOURTH SEMESTER B.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2258 INSTRUMENTAL METHODS OF ANALYSIS LAB L T P C
0 0 4 2
MAIN AIM(S) OF THE COURSE
35
To develop skills of students by providing hands on training in using various equipments
used in biotechnology. This will be a pre-requisite for certain specialized project work
that a student undertakes.
EXPERIMENTS
1. Precision and validity in an experiment using absorption spectroscopy and Validating
Lambert-Beer's law using kMn O4
2. Finding the molar absorbtivity and stoichiometry of the Fe (1, 10 phenanthroline) 3
using absorption spectrometry.
3. Finding the pKa of 4-nirophenol using absorption spectroscopy.
4. UV spectra of nucleic acids.
5. Estimation of Sulphate by nephelometry.
6. Estimation of AL+++ by flourimetry.
7. Chromatography analysis using TLC and Column chromatography.
8. UV spectra of nucleic acids.
9. Limits of detection of colorimeter using aluminum alizam complex.
10. Chromotography using column chromatography.
11. . Job’s plot for funding stochometer of iron salicylate complex.
12. UV – spectra of proteins.
TOTAL: 60 PERIODS
REFERENCE
1. Textbook of Quantitative Inorganic Analysis, AI Vogal, ELBS edition 1987.
LIST OF EQUIPMENTS
1. UV – VIS Spectro photometer, Fluorimeter (optional).
2. TLC chamber (common to biochemistry)
3. Reagents and consumables
4. Measuring cylinders, bathometric flasks of various volumes.
BT2258 INSTRUMENTAL METHODS OF ANALYSIS LAB L T P C
0 0 4 2
MAIN AIM(S) OF THE COURSE
35
To develop skills of students by providing hands on training in using various equipments
used in biotechnology. This will be a pre-requisite for certain specialized project work
that a student undertakes.
EXPERIMENTS
1. Precision and validity in an experiment using absorption spectroscopy and Validating
Lambert-Beer's law using kMn O4
2. Finding the molar absorbtivity and stoichiometry of the Fe (1, 10 phenanthroline) 3
using absorption spectrometry.
3. Finding the pKa of 4-nirophenol using absorption spectroscopy.
4. UV spectra of nucleic acids.
5. Estimation of Sulphate by nephelometry.
6. Estimation of AL+++ by flourimetry.
7. Chromatography analysis using TLC and Column chromatography.
8. UV spectra of nucleic acids.
9. Limits of detection of colorimeter using aluminum alizam complex.
10. Chromotography using column chromatography.
11. . Job’s plot for funding stochometer of iron salicylate complex.
12. UV – spectra of proteins.
TOTAL: 60 PERIODS
REFERENCE
1. Textbook of Quantitative Inorganic Analysis, AI Vogal, ELBS edition 1987.
LIST OF EQUIPMENTS
1. UV – VIS Spectro photometer, Fluorimeter (optional).
2. TLC chamber (common to biochemistry)
3. Reagents and consumables
4. Measuring cylinders, bathometric flasks of various volumes.
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