BT 2257 MICROBIOLOGY LAB SYLLABUS | ANNA UNIVERSITY BTECH BIOTECHNOLOGY 4TH SEM SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FOURTH SEMESTER B.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT 2257 MICROBIOLOGY LAB L T P C
0 0 4 2
AIM(S) OF THE COURSE
The course aims to develop the skills of students in area of microbiology. Here hands on
training is offered for the students to study microbes, their identifications &
characterization and their practical uses.
EXPERIMENTS
1. Laboratory safety and sterilization techniques
2. Microscopic methods in the identification of microorganisms
3. Preparation of culture media – nutrient broth and nutrient agar
4. Culturing of microorganisms – in broth and in plates (pour plates, streak
plates, isolation and preservation of bacterial cultures)
5. Staining techniques – grams’ and differential
6. Quantitation of microorganisms.
7. Effect of disinfectants on microbial flora
8. Isolation and identification of microorganisms from different sources – soil,
water and milk
9. Antibiotic sensitivity assay
10. Growth curve – observation and growth characteristics of bacteria and yeast.
11. Effect of different parameters on bacterial growth (ph, temperature & UV
irradiation)
TOTAL: 60 PERIODS
REFERENCE
1. Micro Biology : Laboratory Theory and applications, M.J. Heboffee aw BE Pierce
Morten Publishing House, 2006.
EQUIPMENTS / APPARATUS
Microbiological Hood for sterilization with UV lighting (One).
Bunsen Burners – 15 Nos.
Orbital Shaker and incubator – 2 Nos.
Refrigerator – 1 No.
Reagents and consumables – Required amount.
BT 2257 MICROBIOLOGY LAB L T P C
0 0 4 2
AIM(S) OF THE COURSE
The course aims to develop the skills of students in area of microbiology. Here hands on
training is offered for the students to study microbes, their identifications &
characterization and their practical uses.
EXPERIMENTS
1. Laboratory safety and sterilization techniques
2. Microscopic methods in the identification of microorganisms
3. Preparation of culture media – nutrient broth and nutrient agar
4. Culturing of microorganisms – in broth and in plates (pour plates, streak
plates, isolation and preservation of bacterial cultures)
5. Staining techniques – grams’ and differential
6. Quantitation of microorganisms.
7. Effect of disinfectants on microbial flora
8. Isolation and identification of microorganisms from different sources – soil,
water and milk
9. Antibiotic sensitivity assay
10. Growth curve – observation and growth characteristics of bacteria and yeast.
11. Effect of different parameters on bacterial growth (ph, temperature & UV
irradiation)
TOTAL: 60 PERIODS
REFERENCE
1. Micro Biology : Laboratory Theory and applications, M.J. Heboffee aw BE Pierce
Morten Publishing House, 2006.
EQUIPMENTS / APPARATUS
Microbiological Hood for sterilization with UV lighting (One).
Bunsen Burners – 15 Nos.
Orbital Shaker and incubator – 2 Nos.
Refrigerator – 1 No.
Reagents and consumables – Required amount.
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