BM 2258 PATHOLOGY AND MICROBIOLOGY LAB SYLLABUS | ANNA UNIVERSITY BE BIOMEDICAL ENGINEERING 1ST SEM SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER BE BIOMEDICAL ENGINEERING DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BM 2258 PATHOLOGY AND MICROBIOLOGY LAB L T P C
0 0 4 2
1. Urine physical and chemical examination (protein, reducing substances, ketones,
bilirubin and blood)
2. Hematoxylin and eosin staining.
3. Study of parts of compound microscope
4. Histopathological slides of benign and malignant tumours.
5. Manual tissue processing and section cutting (demonstration)
6. Simple stain.
7. Gram stain.
8. AFB stain.
9. Slides of malyarial parasites, micro filaria and leishmania donovani.
10. Haematology slides of anemia and leukemia
11. Bleeding time and clotting time.
12. Study of bone marrow charts
TOTAL: 60 PERIODS
44
(Lab Requirement for a both of Semester)
1) Test tubes, Test tube racks, Bunsen burner(gas),(Benedict’s solution, Acetic acid,
Sulphosalicylic acid), centrifuge, microscope.
2) Slides, cover slips,H & E stains
3) Microscope
4) Beakers,graded alcohols, acetone,Lmoulds(or maling bodies), paradigm wax, water
bath, microtone for section cutting,slides,cover slips,hotair oven,refrigerator to store
chemical and ice.
5) whanman filter paper for bleeding time Test tubes.
6) Simple Stain test
SIMPLE STAIN, Glass Slide Reagents – Methylene blue, Crystal Violet, Cabal Iuschin
7) Gram Stain test SIMPLE GRAM STAIN
Reagents – Crystal Violet, Gram’s Iodine, 95% ethyl alcohol Safranin
Urine bacterial count/ml exceeding 100,000(10s) denotes urinary tract infection.
Normal : 0-100 ml Glass Slide
8) AFB Strain test ACID FAST STAIN (ZIEHL – NEELSON METHOD) Reagent – Carbal
Iushin, Acid Alcohol, Methylene blue, Glass slide
9) Bone marrow charts
BM 2258 PATHOLOGY AND MICROBIOLOGY LAB L T P C
0 0 4 2
1. Urine physical and chemical examination (protein, reducing substances, ketones,
bilirubin and blood)
2. Hematoxylin and eosin staining.
3. Study of parts of compound microscope
4. Histopathological slides of benign and malignant tumours.
5. Manual tissue processing and section cutting (demonstration)
6. Simple stain.
7. Gram stain.
8. AFB stain.
9. Slides of malyarial parasites, micro filaria and leishmania donovani.
10. Haematology slides of anemia and leukemia
11. Bleeding time and clotting time.
12. Study of bone marrow charts
TOTAL: 60 PERIODS
44
(Lab Requirement for a both of Semester)
1) Test tubes, Test tube racks, Bunsen burner(gas),(Benedict’s solution, Acetic acid,
Sulphosalicylic acid), centrifuge, microscope.
2) Slides, cover slips,H & E stains
3) Microscope
4) Beakers,graded alcohols, acetone,Lmoulds(or maling bodies), paradigm wax, water
bath, microtone for section cutting,slides,cover slips,hotair oven,refrigerator to store
chemical and ice.
5) whanman filter paper for bleeding time Test tubes.
6) Simple Stain test
SIMPLE STAIN, Glass Slide Reagents – Methylene blue, Crystal Violet, Cabal Iuschin
7) Gram Stain test SIMPLE GRAM STAIN
Reagents – Crystal Violet, Gram’s Iodine, 95% ethyl alcohol Safranin
Urine bacterial count/ml exceeding 100,000(10s) denotes urinary tract infection.
Normal : 0-100 ml Glass Slide
8) AFB Strain test ACID FAST STAIN (ZIEHL – NEELSON METHOD) Reagent – Carbal
Iushin, Acid Alcohol, Methylene blue, Glass slide
9) Bone marrow charts
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