BT2407 DOWNSTREAM PROCESSING LAB SYLLABUS | ANNA UNIVERSITY B.TECH BIOTECHNOLOGY 7TH SEM SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SEVENTH SEMESTER B.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2407 DOWNSTREAM PROCESSING LAB L T P C
0 0 4 2
AIM
To provide hands on training in Down stream processing by through simple
experimentation in the laboratory. This will be a pre-requisite for project work.
OBJECTIVES
At the end of the course, the student has gained the knowledge to perform various
techniques used in Down Stream Processing and how to make a finished project.
1.Solid liquid separation – centrifugation, microfiltration
2.Cell disruption techniques – ultrasonication, French pressure cell
3.Cell disruption techniques – dyno mill – batch and continuous
4.Precipitation – ammonium sulphite precipitation
5.Ultra filtration separation
6.Aqueous two phase extraction of biologicals
7.High resolution purification – affinity chromatography
8.High resolution purification – ion exchange chromatography
9.Product polishing – gel filtration chromatography
10.10.Product polishing spray drying freeze drying
TOTAL : 60 PERIODS
BT2407 DOWNSTREAM PROCESSING LAB L T P C
0 0 4 2
AIM
To provide hands on training in Down stream processing by through simple
experimentation in the laboratory. This will be a pre-requisite for project work.
OBJECTIVES
At the end of the course, the student has gained the knowledge to perform various
techniques used in Down Stream Processing and how to make a finished project.
1.Solid liquid separation – centrifugation, microfiltration
2.Cell disruption techniques – ultrasonication, French pressure cell
3.Cell disruption techniques – dyno mill – batch and continuous
4.Precipitation – ammonium sulphite precipitation
5.Ultra filtration separation
6.Aqueous two phase extraction of biologicals
7.High resolution purification – affinity chromatography
8.High resolution purification – ion exchange chromatography
9.Product polishing – gel filtration chromatography
10.10.Product polishing spray drying freeze drying
TOTAL : 60 PERIODS
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