BT2401 DOWNSTREAM PROCESSING SYLLABUS | ANNA UNIVERSITY B.TECH BIOTECHNOLOGY 7TH SEM SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY SEVENTH SEMESTER B.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2401 DOWNSTREAM PROCESSING L T P C
3 1 0 4
AIM
This course aims to develop the skills of the students in the area of Downstream
processing. This is a pre-requisite for courses in Bioprocess Technology.
OBJECTIVES
At the end of the course, the student would have learnt about ,methods to obtain
pure proteins, enzymes and in general about product development R & D. This will
be handy for projects of Industries.
UNIT I DOWNSTREAM PROCESSING 8+3
Introduction to downstream processing principles characteristics of biomolecules and
bioprocesses. Cell disruption for product release – mechanical, enzymatic and chemical
methods. Pretreatment and stabilisation of bioproducts.
UNIT II PHYSICAL METHODS OF SEPERATION 6+3
Unit operations for solid-liquid separation - filtration and centrifugation.
UNIT III ISOLATION OF PRODUCTS 12+3
Adsorption, liquid-liquid extraction, aqueous two-phase extraction, membrane separation
– ultrafiltration and reverse osmosis, dialysis, precipitation of proteins by different
methods.
UNIT IV PRODUCT PURIFICATION 12+3
Chromatography – principles, instruments and practice, adsorption, reverse phase, ionexchange,
size exclusion, hydrophobic interaction, bioaffinity and pseudo affinity
chromatographic techniques.
UNIT V FINAL PRODUCT FORMULATION AND FINISHING OPERATIONS 7+3
Crystallization, drying and lyophilization in final product formulation.
L : 45 , T : 15 ,TOTAL : 60 PERIODS
TEXT BOOKS
1. P.A. Belter, E.L. Cussler And Wei-Houhu – Bioseparations – Downstream
Processing For Biotechnology, Wiley Interscience Pub. (1988).
2. R.O. Jenkins, (Ed.) – Product Recovery In Bioprocess Technology – Biotechnology
By Open Learning Series, Butterworth-Heinemann (1992).
REFERENCES
1. J.C. Janson And L. Ryden, (Ed.) – Protein Purification – Principles, High Resolution
Methods And Applications, VCH Pub. 1989.
2. R.K. Scopes – Protein Purification – Principles And Practice, Narosa Pub. (1994).
3. Roger.G . Harrison , Paul Todd , Scott R.Rudge and Demetri P.Petrides,
Bioseperation Science and Engineering , Oxford University Press , Newyork , 2003.
BT2401 DOWNSTREAM PROCESSING L T P C
3 1 0 4
AIM
This course aims to develop the skills of the students in the area of Downstream
processing. This is a pre-requisite for courses in Bioprocess Technology.
OBJECTIVES
At the end of the course, the student would have learnt about ,methods to obtain
pure proteins, enzymes and in general about product development R & D. This will
be handy for projects of Industries.
UNIT I DOWNSTREAM PROCESSING 8+3
Introduction to downstream processing principles characteristics of biomolecules and
bioprocesses. Cell disruption for product release – mechanical, enzymatic and chemical
methods. Pretreatment and stabilisation of bioproducts.
UNIT II PHYSICAL METHODS OF SEPERATION 6+3
Unit operations for solid-liquid separation - filtration and centrifugation.
UNIT III ISOLATION OF PRODUCTS 12+3
Adsorption, liquid-liquid extraction, aqueous two-phase extraction, membrane separation
– ultrafiltration and reverse osmosis, dialysis, precipitation of proteins by different
methods.
UNIT IV PRODUCT PURIFICATION 12+3
Chromatography – principles, instruments and practice, adsorption, reverse phase, ionexchange,
size exclusion, hydrophobic interaction, bioaffinity and pseudo affinity
chromatographic techniques.
UNIT V FINAL PRODUCT FORMULATION AND FINISHING OPERATIONS 7+3
Crystallization, drying and lyophilization in final product formulation.
L : 45 , T : 15 ,TOTAL : 60 PERIODS
TEXT BOOKS
1. P.A. Belter, E.L. Cussler And Wei-Houhu – Bioseparations – Downstream
Processing For Biotechnology, Wiley Interscience Pub. (1988).
2. R.O. Jenkins, (Ed.) – Product Recovery In Bioprocess Technology – Biotechnology
By Open Learning Series, Butterworth-Heinemann (1992).
REFERENCES
1. J.C. Janson And L. Ryden, (Ed.) – Protein Purification – Principles, High Resolution
Methods And Applications, VCH Pub. 1989.
2. R.K. Scopes – Protein Purification – Principles And Practice, Narosa Pub. (1994).
3. Roger.G . Harrison , Paul Todd , Scott R.Rudge and Demetri P.Petrides,
Bioseperation Science and Engineering , Oxford University Press , Newyork , 2003.
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