IB3307 MOLECULAR BIOLOGY LAB SYLLABUS | ANNA UNIVERSITY B.TECH. FOOD TECHNOLOGY 5TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIFTH SEMESTER B.TECH. FOOD TECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS, IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
IB3307 MOLECULAR BIOLOGY LAB L T P C
0 0 4 2
1. Isolation of bacterial DNA
2. Isolation of plant cell and animal cell genomic DNA
3. Agarose gel electrophoresis
4. Restriction enzyme digestion
5. Competent cells preparation
6. Transformation and screening for recombinants
7. Agarose gel electrophoresis
8. Restriction enzyme digestion
9. Competent cells preparation
10. Blue and white selection for recombinants
11. Plating of phage
12. phage lysis of liquid cultures
TOTAL: 60 PERIODS
REFERENCE
1. Ausubel, F.M. “Short Protocols in Molecular Biology”, 4th Edition, John Wiley, 1999.
IB3307 MOLECULAR BIOLOGY LAB L T P C
0 0 4 2
1. Isolation of bacterial DNA
2. Isolation of plant cell and animal cell genomic DNA
3. Agarose gel electrophoresis
4. Restriction enzyme digestion
5. Competent cells preparation
6. Transformation and screening for recombinants
7. Agarose gel electrophoresis
8. Restriction enzyme digestion
9. Competent cells preparation
10. Blue and white selection for recombinants
11. Plating of phage
12. phage lysis of liquid cultures
TOTAL: 60 PERIODS
REFERENCE
1. Ausubel, F.M. “Short Protocols in Molecular Biology”, 4th Edition, John Wiley, 1999.
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