BT2307 MOLECULAR BIOLOGY LAB SYLLABUS | ANNA UNIVERSITY B.TECH. BIOTECHNOLOGY 5TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIFTH SEMESTER B.TECH. BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS, IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2307 MOLECULAR BIOLOGY LAB L T P C
0 0 4 2
AIM
To develop the skills of the students by providing hands on training practical training in
Molecular Biology. This will facilitate the students to take up specialized project in
Molecular biology and will be a pre-requisite for research work.
OBJECTIVES
At the end of this course, the students would have learnt basic techniques used in
Molecular Biology and its application. This will be strength for students to undertake
research projects in the area of moderabiology.
1. Isolation of bacterial DNA
2. Isolation of plant cell and animal cell genomic DNA
3. Agarose gel electrophoresis
4. Restriction enzyme digestion
5. Competent cells preparation
6. Transformation and screening for recombinants
7. Agarose gel electrophoresis
8. Restriction enzyme digestion
9. Competent cells preparation
10. Blue and white selection for recombinants
11. Plating of Ophage
12. O phage lysis of liquid cultures
TOTAL : 60 PERIODS
BT2307 MOLECULAR BIOLOGY LAB L T P C
0 0 4 2
AIM
To develop the skills of the students by providing hands on training practical training in
Molecular Biology. This will facilitate the students to take up specialized project in
Molecular biology and will be a pre-requisite for research work.
OBJECTIVES
At the end of this course, the students would have learnt basic techniques used in
Molecular Biology and its application. This will be strength for students to undertake
research projects in the area of moderabiology.
1. Isolation of bacterial DNA
2. Isolation of plant cell and animal cell genomic DNA
3. Agarose gel electrophoresis
4. Restriction enzyme digestion
5. Competent cells preparation
6. Transformation and screening for recombinants
7. Agarose gel electrophoresis
8. Restriction enzyme digestion
9. Competent cells preparation
10. Blue and white selection for recombinants
11. Plating of Ophage
12. O phage lysis of liquid cultures
TOTAL : 60 PERIODS
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